Analysis of Mutations in the Pma1 Plasma Membrane Atpase Proton Pump That Suppress a Temperature Sensitive Growth Defect Phenotype Of Vacuolar Membrane Atpase Proton Pump Deficient Saccharomyces Cerevisiae

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dc.contributor.advisorWasko, Brian
dc.contributor.advisorStephens, Brian
dc.contributor.committeeMemberRashid, Bazlur
dc.creatorPylypchuk, Kateryna
dc.date.accessioned2024-07-02T15:27:19Z
dc.date.available2024-07-02T15:27:19Z
dc.date.created2022-05
dc.date.issued2022-05-12
dc.date.submittedMay 2022
dc.date.updated2024-07-02T15:27:20Z
dc.description.abstractThe P-type proton pump, Pma1p and the vacuolar H+-ATPase (V-ATPase) proton pump play significant roles in balancing pH homeostasis in eukaryotic cells. In budding yeast, the PMA1 gene is critical for yeast survival, whereas mutations compromising V-ATPase activity cause conditional lethality. The yeast VMA21 gene encodes for the chaperone protein Vma21 that is required for complete assembly of the multisubunit V-ATPase complex. The loss of vacuolar acidity due to deficiency of V-ATPase activity has been linked to disrupted growth phenotypes. Yeast vma21 deficient mutants exhibit sensitivity to cold temperatures, CaCl2 and acetic acid. This sensitivity can be suppressed by a Pma1-G158S mutation suggesting an interdependence between Pma1p and the V-ATPase. In this study, we address how Pma1-G158S mutation affects wildtype and V-ATPase deficient cells in response to changes in extracellular conditions. Using CRISPR/Cas9 gene editing technology, we performed site-directed mutagenesis of G158 in an attempt to analyze structure-function relationships at this amino acid position. Our observations suggest that mutations at this position may regulate aging phenotype and cellular dysfunctions in yeast Saccharomyces cerevisiae and multicellular eukaryotes.  
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/10657.1/4960
dc.language.isoen
dc.subjectDNA damage
dc.subjectVacuolar acidity
dc.subjectSaccharomyces cerevisiae
dc.subjectProton pump
dc.subjectVacuolar ATPase
dc.subjectpH homeostasis
dc.titleAnalysis of Mutations in the Pma1 Plasma Membrane Atpase Proton Pump That Suppress a Temperature Sensitive Growth Defect Phenotype Of Vacuolar Membrane Atpase Proton Pump Deficient Saccharomyces Cerevisiae
dc.typeThesis
dc.type.materialtext
thesis.degree.grantorUniversity of Houston-Clear Lake
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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