Now showing items 11-20 of 23
Effects of lysine, threonine, and methionine on light-driven protein synthesis in isolated pea (Pisum sativum L.) chloroplasts
Light-driven incorporation of [14C]leucine (LEU) into protein in isolated pea chloroplasts was inhibited by 0.1 and 1 mM lysine (LYS), 0.1–10 mM threonine (THR) and 1 mM methionine (MET). Equimolar combinations of LYS plus ...
Amino acid biosynthesis in isolated pea chloroplasts: metabolism of labeled aspartate and sulfate
(FEBS Letters, 1978)
Accumulating evidence points to chloroplasts as the site of nitrite and sulfate reduction and incorporation into carbon skeletons [I] Furthermore, several enzymes of aspartate-family amino acid biosynthesis recently have ...
Identification and measurement of homoserine by gas-liquid chromatography
(Analytical Biochemistry, 1980)
Homoserine has been analyzed quantitatively by gas-liquid chromatography of its N-heptafluorobutyryl isopropyl ester. The method was confirmed by analysis of the soluble amino acid fraction of pea leaves. The possible use ...
Resensitization to gibberellin of grand rapids lettuce seeds treated with continuous far red light
(Journal of the Tennessee Academy of Science, 1973)
A rapid method for isolation of purified physiologically active chloroplasts, used to study the intracellular distribution of amino acids in pea leaves
A procedure is described for the rapid (<5 min) isolation of purified, physiologically active chloroplasts from Pisum sativum L. Mitochondrial and microbody contamination is substantially reduced and broken chloroplasts ...
Purification of chloroplasts using silica-sols
(Academic Press, 1987)
This chapter describes procedures for the purification of chloroplasts using silica sols. Silica sols have two major advantages as media for density gradients—namely, low viscosity and negligible osmotic potential, allowing ...
Light-driven DNA biosynthesis in isolated pea chloroplasts
(FEBS Letters, 1983)
Isolated chloroplasts from Pisum sativum leaves catalyze [3H]thymidine incorporation into acid-insoluble material using light as the sole energy source. Neither ribonucleotides, deoxyribonucleotides nor deoxyribonucleosides ...
ß- alanine metabolism and high salinity stress in the sea anemone, Bunodosoma cavernata
(Journal of Comparative Physiology B, 1984)
During high salinity stress, β-alanine accumulates to high levels in the sea anemone,Bunodosoma cavernata. Following a salinity increase from 26‰ to 40‰ β-alanine increased 28-fold from 1.5 to 41.9 μmoles/g dry weight. ...
Photosynthetic formation of the aspartate family of amino acids in isolated chloroplasts
(Plant Physiology, 1980)
The metabolism of 14C-labeled aspartic acid, diaminopimelic acid, malic acid and threonine by isolated pea (Pisum sativum L.) chloroplasts was examined. Light enhanced the incorporation of [14C] aspartic acid into soluble ...