Now showing items 1-4 of 4
Intracellular formation of "undisruptable" dimers of inducible nitric oxide synthase
(Proc Natl Acad Sci U S A, 2003-11-25)
Overproduction of nitric oxide (NO) by inducible NO synthase (iNOS) has been implicated in the pathogenesis of many diseases. iNOS is active only as a homodimer. Dimerization of iNOS represents a potentially critical target ...
Characterization of key residues in the subdomain encoded by exons 8 and 9 of human inducible nitric oxide synthase: a critical role for Asp-280 in substrate binding and subunit interactions
(Proc Natl Acad Sci U S A, 2001-08-28)
Human inducible nitric oxide synthase (iNOS) is active as a dimer of two identical subunits. Each subunit has an amino-terminal oxygenase domain that binds the substrate l-Arg and the cofactors heme and tetrahydrobiopterin ...
Cysteine-less GPI-phospholipase C is Inhibited Competitively by a Sulfhydryl Reagent: Glyco-mimicry by para-Chloromercuriphenylsulfonate
(Biochem J., 2002-08-15)
Glycosylphosphatidylinositol (GPI)-specific phospholipases are highly valuable for studying the structure and function of GPIs. GPI-specific phospholipase C (GPI-PLC) from Trypanosoma brucei and phosphatidylinositol-specific ...
A novel kanamycin/neomycin phosphotransferase cassette increases transformation efficiency of E. coli
Stable transformation depends on the efficient delivery of DNA into cells and the robust expression of genes that encode proteins which provide resistance to selective (cytotoxic) compounds. We have examined the possibility ...