During leaf development, chloroplasts are formed by the binary fission of pre-existing plastids (1); thus, replication of chloroplast DNA (ctDNA) must occur to maintain an adequate amount in each organelle. The levels of ...

This chapter describes procedures for the purification of chloroplasts using silica sols. Silica sols have two major advantages as media for density gradients—namely, low viscosity and negligible osmotic potential, allowing ...