Now showing items 1-10 of 16
DNA synthesis in chloroplasts. I. Characterization of and developmental variation in both light- and ATP-driven thymidine incorporation by isolated intact chloroplasts
(Journal of Experimental Botany, 1988)
Intact chloroplasts from young pea leaves were able to incorporate [3H]thymidine into DNA at relatively high rates (50 pmol mg-1 chlorophyll h-1 or more), using light as the sole energy source. The intact plastids were ...
A rapid method for isolation of purified physiologically active chloroplasts, used to study the intracellular distribution of amino acids in pea leaves
A procedure is described for the rapid (<5 min) isolation of purified, physiologically active chloroplasts from Pisum sativum L. Mitochondrial and microbody contamination is substantially reduced and broken chloroplasts ...
Synthesis of aspartate-family amino acids
(CRC Press, 1987)
DNA synthesis in isolated chloroplasts
(Cambridge University Press, 1988)
Identification and measurement of homoserine by gas-liquid chromatography
(Analytical Biochemistry, 1980)
Homoserine has been analyzed quantitatively by gas-liquid chromatography of its N-heptafluorobutyryl isopropyl ester. The method was confirmed by analysis of the soluble amino acid fraction of pea leaves. The possible use ...
DNA synthesis in chloroplasts. III. The DNA gyrase inhibitors nalidixic acid and novobiocin inhibit both thymidine incorporation into DNA and photosynthetic oxygen evolution in isolated chloroplasts
(Journal of Experimental Botany, 1989)
The influence of two DNA gyrase inhibitors, nalidixic acid and novobiocin, on DNA synthesis in isolated pea chloroplasts was examined. Novobiocin at 1–5 mol m−3 markedly lowered [3H]thymidine incorporation into DNA (30–95% ...
Photosynthetic formation of the aspartate family of amino acids in isolated chloroplasts
(Plant Physiology, 1980)
The metabolism of 14C-labeled aspartic acid, diaminopimelic acid, malic acid and threonine by isolated pea (Pisum sativum L.) chloroplasts was examined. Light enhanced the incorporation of [14C] aspartic acid into soluble ...
DNA biosynthesis in chloroplasts and its regulation: Studies on isolated chloroplasts and chloroplast extracts
(Progress in Photosynthesis Research, 1987)
During leaf development, chloroplasts are formed by the binary fission of pre-existing plastids (1); thus, replication of chloroplast DNA (ctDNA) must occur to maintain an adequate amount in each organelle. The levels of ...
DNA biosynthesis in intact, isolated chloroplasts
(Clarendon Press, 1984)