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Buffering the pH of the Culture Medium does not Extend Yeast Replicative Lifespan
(F1000 Research, 2013)
During chronological aging of the budding yeast cells, the culture medium can become acidified and this acidification limits cell survival. As a consequence, buffering the culture medium to pH 6 significantly extends ...
Inhibition of DNA double-strand break repair by the Ku heterodimer in mrx mutants of Saccharomyces cerevisiae
(DNA Repair, 2009)
Yeast rad50 and mre11 nuclease mutants are hypersensitive to physical and chemical agents that induce DNA double-strand breaks (DSBs). This sensitivity was suppressed by elevating intracellular levels of TLC1, the RNA ...
Nar1 Deficiency Results in Shortened Lifespan and Sensitivity to Paraquat that is Rescued by Increased Expression of Mitochondrial Superoxide Dismutase
(Mech Ageing Dev, 2014)
Saccaromyces cerevisiae Nar lp is an essential Fe/S protein that exhibits striking similarity to bacterial iron-only hydogenases. Nar1p is required for the maturation of cytosolic and nuclear, but not of mitochondrial ...
Quantitative determination of isopentenyl diphosphate in cultured mammalian cells
(Analytical Biochemistry, 2012)
Isopentenyl diphosphate (IPP), an intermediate of the isoprenoid biosynthetic pathway (IBP), has several important biological functions, yet a method to determine its basal level has not been described. Here, we describe ...
Molecular Mechanisms Underlying Genotype-dependent Responses to Dietary Restrictions
(Aging Cell, 2013)
Dietary restriction (DR) increases lifespan and attenuates age-related phenotypes in many organisms; however, the effects of DR on longevity of individuals in genetically heterogeneous populations is not well characterized. ...
mTOR Inhibition by Rapamycin Alleviates Mitochondrial Disease in the Ndufs4-Mouse Model of Leigh Syndrome
(Science, 2013)
Mitochondrial dysfunction contributes to numerous health problems, including neurological and muscular degeneration, cardiomyopathies, cancer, diabetes, and pathologies of aging. Severe mitochondrial defects can result ...
Defining Molecular Basis for the Longevity Traits in Natural Yeast Isolates
(NPJ Aging Mechanisms of Disease, 2015)
Background:
The budding yeast has served as a useful model organism in aging studies, leading to the identification of genetic determinants of longevity, many of which are conserved in higher eukaryotes. However, factors ...
Restoring Membrane Protein Trafficking Rescues the Effects of Aneuploidy on Cellular Aging
(2016)
Aneuploidy and aging are correlated; however, a causal link between these two phenomena has remained elusive. Here, we show that yeast disomic for a single native yeast chromosome generally have a decreased replicative ...
Defining the Impact of Mutation Accumulation on Replicative Lifespan in Yeast Using Cancer-associated Mutator Phenotypes
(PNAS, 2019)
Mutations accumulate within somatic cells and have been proposed to contribute to aging. It is unclear what level of mutation burden may be required to consistently reduce cellular lifespan. Humans cancers driven by a ...
pH Neutralization Protects Against Reduction in Replicative Lifespan Following Chronological Aging in Yeast
(Cell Cycle, 2012)
Chronological and replicative aging have been studied in yeast as alternative paradigms for post-mitotic and mitotic aging, respectively. It has been known for more than a decade that cells of the S288C background aged ...