Show simple item record

dc.contributor.authorWasko, Brian
dc.date.accessioned2021-02-02T17:53:11Z
dc.date.available2021-02-02T17:53:11Z
dc.date.issued2013
dc.identifier.citationWasko BM, Carr DT, Tung H, Doan H, Schurman N, Neault JR, Feng J, Lee J, Zipkin B, Mouser J, Oudanonh E, Nguyen T, Stetina T, Shemorry A, Lemma M, Kaeberlein M. Buffering the pH of the culture medium does not extend yeast replicative lifespan. F1000Res. 2013, Oct 15;2:216en_US
dc.identifier.urihttps://hdl.handle.net/10657.1/2554
dc.description.abstractDuring chronological aging of the budding yeast cells, the culture medium can become acidified and this acidification limits cell survival. As a consequence, buffering the culture medium to pH 6 significantly extends chronological life span under standard conditions in synthetic medium. In this study, we assessed whether a similar process occur during replicative aging of yeast cells. We find no evidence that buffering the pH of the culture medium to pH levels either higher or lower than the initial pH of the medium is able to significantly extend replicative lifespan. Thus we conclude that, unlike chronological life span, replicative life span is not limited by acidification of the culture medium or by changes in the pH of the environment.en_US
dc.publisherF1000 Researchen_US
dc.subjectculture medium, pH, replicative aging, bufferingen_US
dc.titleBuffering the pH of the Culture Medium does not Extend Yeast Replicative Lifespanen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record