Browsing W. Ronald Mills by Title
Now showing items 12-23 of 23
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Identification and measurement of homoserine by gas-liquid chromatography
(Analytical Biochemistry, 1980)Homoserine has been analyzed quantitatively by gas-liquid chromatography of its N-heptafluorobutyryl isopropyl ester. The method was confirmed by analysis of the soluble amino acid fraction of pea leaves. The possible use ... -
The isolation of a lysine sensitive aspartate kinase from pea leaves and its involvement in homoserine biosynthesis in isolated chloroplasts
(FEBS Letters, 1979)Homoserine is an intermediate in the biosynthesis of the essential amino acids Thr and Met in microorganisms [I] and higher plants [2]. Pea seedlings synthesise massive amounts of Hse, mainly in the roots [3-51, although ... -
Itracellular localization of aspartate kinase in spinach (Spinacea oleracea)
(FEBS Letters, 1979)1. Enzyme distribution between chloroplasts and the nonchloroplast parts of green leaf cells of Spinacia oleracea, Nicotiana rustica, Vicia faba, and Phaseolus vulgaris have been investigated by use of the nonaqueous ... -
Light-driven DNA biosynthesis in isolated pea chloroplasts
(FEBS Letters, 1983)Isolated chloroplasts from Pisum sativum leaves catalyze [3H]thymidine incorporation into acid-insoluble material using light as the sole energy source. Neither ribonucleotides, deoxyribonucleotides nor deoxyribonucleosides ... -
Photosynthetic formation of the aspartate family of amino acids in isolated chloroplasts
(Plant Physiology, 1980)The metabolism of 14C-labeled aspartic acid, diaminopimelic acid, malic acid and threonine by isolated pea (Pisum sativum L.) chloroplasts was examined. Light enhanced the incorporation of [14C] aspartic acid into soluble ... -
Purification of chloroplasts using silica-sols
(Academic Press, 1987)This chapter describes procedures for the purification of chloroplasts using silica sols. Silica sols have two major advantages as media for density gradients—namely, low viscosity and negligible osmotic potential, allowing ... -
A rapid method for isolation of purified physiologically active chloroplasts, used to study the intracellular distribution of amino acids in pea leaves
(Planta, 1980)A procedure is described for the rapid (<5 min) isolation of purified, physiologically active chloroplasts from Pisum sativum L. Mitochondrial and microbody contamination is substantially reduced and broken chloroplasts ... -
Regulation of aspartate-family amino acid biosynthesis: studies using isolated pea chloroplasts
(Kluwer Academic Publishers, 1993) -
Resensitization to gibberellin of grand rapids lettuce seeds treated with continuous far red light
(Journal of the Tennessee Academy of Science, 1973) -
Synthesis of aspartate-family amino acids
(CRC Press, 1987) -
Synthesis of branched chain amino acids
(CRC Press, 1987) -
ß- alanine metabolism and high salinity stress in the sea anemone, Bunodosoma cavernata
(Journal of Comparative Physiology B, 1984)During high salinity stress, β-alanine accumulates to high levels in the sea anemone,Bunodosoma cavernata. Following a salinity increase from 26‰ to 40‰ β-alanine increased 28-fold from 1.5 to 41.9 μmoles/g dry weight. ...
