W. Ronald Mills
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Dr. Mills is a Professor of Biology and Chemistry at College of Science and Engineering in University of Houston-Clear Lake.
Recent Submissions
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Regulation of aspartate-family amino acid biosynthesis: studies using isolated pea chloroplasts
(Kluwer Academic Publishers, 1993) -
Developmental variation in aspartate-family amino acid biosynthesis by isolated chloroplasts
(Kluwer Academic Publishers, 1990)In the last decade, it has become clear that the chloroplast is the main site, if not the sole site, for the biosynthesis of the nutritionally essential aspartate-derived amino acids (Fig. 1) in plant leaves [1,2]. For ... -
DNA synthesis in chloroplasts. III. The DNA gyrase inhibitors nalidixic acid and novobiocin inhibit both thymidine incorporation into DNA and photosynthetic oxygen evolution in isolated chloroplasts
(Journal of Experimental Botany, 1989)The influence of two DNA gyrase inhibitors, nalidixic acid and novobiocin, on DNA synthesis in isolated pea chloroplasts was examined. Novobiocin at 1–5 mol m−3 markedly lowered [3H]thymidine incorporation into DNA (30–95% ... -
DNA synthesis in isolated chloroplasts
(Cambridge University Press, 1988) -
DNA synthesis in chloroplasts. II. Developmental variation in TTP incorporation into DNA by plastid extracts
(Journal of Experimental Botany, 1988)Incorporation of [3H]TTP into DNA by pea chloroplast extracts was highly dependent on the age of the tissue from which plastids were prepared. Catalytic activity was greatest in samples from 6- to 9-d-old plants; preparations ... -
DNA synthesis in chloroplasts. I. Characterization of and developmental variation in both light- and ATP-driven thymidine incorporation by isolated intact chloroplasts
(Journal of Experimental Botany, 1988)Intact chloroplasts from young pea leaves were able to incorporate [3H]thymidine into DNA at relatively high rates (50 pmol mg-1 chlorophyll h-1 or more), using light as the sole energy source. The intact plastids were ... -
Synthesis of aspartate-family amino acids
(CRC Press, 1987) -
Synthesis of branched chain amino acids
(CRC Press, 1987) -
DNA biosynthesis in intact, isolated chloroplasts
(Clarendon Press, 1984) -
DNA biosynthesis in chloroplasts and its regulation: Studies on isolated chloroplasts and chloroplast extracts
(Progress in Photosynthesis Research, 1987)During leaf development, chloroplasts are formed by the binary fission of pre-existing plastids (1); thus, replication of chloroplast DNA (ctDNA) must occur to maintain an adequate amount in each organelle. The levels of ... -
Purification of chloroplasts using silica-sols
(Academic Press, 1987)This chapter describes procedures for the purification of chloroplasts using silica sols. Silica sols have two major advantages as media for density gradients—namely, low viscosity and negligible osmotic potential, allowing ... -
ß- alanine metabolism and high salinity stress in the sea anemone, Bunodosoma cavernata
(Journal of Comparative Physiology B, 1984)During high salinity stress, β-alanine accumulates to high levels in the sea anemone,Bunodosoma cavernata. Following a salinity increase from 26‰ to 40‰ β-alanine increased 28-fold from 1.5 to 41.9 μmoles/g dry weight. ... -
Amino acid biosynthesis in chloroplasts
(Balaban International Science Service, 1981) -
Light-driven DNA biosynthesis in isolated pea chloroplasts
(FEBS Letters, 1983)Isolated chloroplasts from Pisum sativum leaves catalyze [3H]thymidine incorporation into acid-insoluble material using light as the sole energy source. Neither ribonucleotides, deoxyribonucleotides nor deoxyribonucleosides ... -
Photosynthetic formation of the aspartate family of amino acids in isolated chloroplasts
(Plant Physiology, 1980)The metabolism of 14C-labeled aspartic acid, diaminopimelic acid, malic acid and threonine by isolated pea (Pisum sativum L.) chloroplasts was examined. Light enhanced the incorporation of [14C] aspartic acid into soluble ... -
A rapid method for isolation of purified physiologically active chloroplasts, used to study the intracellular distribution of amino acids in pea leaves
(Planta, 1980)A procedure is described for the rapid (<5 min) isolation of purified, physiologically active chloroplasts from Pisum sativum L. Mitochondrial and microbody contamination is substantially reduced and broken chloroplasts ... -
Identification and measurement of homoserine by gas-liquid chromatography
(Analytical Biochemistry, 1980)Homoserine has been analyzed quantitatively by gas-liquid chromatography of its N-heptafluorobutyryl isopropyl ester. The method was confirmed by analysis of the soluble amino acid fraction of pea leaves. The possible use ... -
Resensitization to gibberellin of grand rapids lettuce seeds treated with continuous far red light
(Journal of the Tennessee Academy of Science, 1973) -
Itracellular localization of aspartate kinase in spinach (Spinacea oleracea)
(FEBS Letters, 1979)1. Enzyme distribution between chloroplasts and the nonchloroplast parts of green leaf cells of Spinacia oleracea, Nicotiana rustica, Vicia faba, and Phaseolus vulgaris have been investigated by use of the nonaqueous ...
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