DNA biosynthesis in chloroplasts and its regulation: Studies on isolated chloroplasts and chloroplast extracts

Date

1987

Authors

Mills, Ronald

Journal Title

Journal ISSN

Volume Title

Publisher

Progress in Photosynthesis Research

Abstract

During leaf development, chloroplasts are formed by the binary fission of pre-existing plastids (1); thus, replication of chloroplast DNA (ctDNA) must occur to maintain an adequate amount in each organelle. The levels of ctDNA in plastids vary during plant development (2); the fraction of chloroplast DNA increases during early shoot development then stabilizes thereafter. The number of genome copies per plastid also increases early, however, they reach a maximum then decline as tissues mature. Such variation in the levels of ctDNA suggests that the capacity of chloroplasts to replicate their DNA changes during development. Here, we describe the use of both isolated intact pea chloroplasts and plastid extracts to study developmental changes in ctDNA biosynthesis.

Description

Keywords

Thymidine Incorporation, Light Control, Intact Chloroplast, Binary Fission, Oxolinic Acid

Citation

Mills, W. R., and B. J. Baumgartner. 1987. DNA biosynthesis in chloroplasts and its regulation: Studies on isolated chloroplasts and chloroplast extracts. Progress in Photosynthesis Research. 4, 683- 686.